BEMÆRK: Ansøgningsfristen er overskredet
Cell culture is a cornerstone of biomedical research and increasingly, mammalian cells, such as stem-, immune- and cancer cells, are used for therapy, precision medicine and toxicology. Surprisingly, we know very little about how these cells utilize the different components of the medium they grow in. Organ on a chip with its improved mass transfer in dynamic cultures furthermore challenge current practices for cell cultures.
We are looking for a candidate that are interested in understanding how cells use medium components during culture. The candidate will get experience with cancer and stem cell culture and differentiation and analysis of the medium usage pattern using NMR and immunoassays. The knowledge will be used to optimize mediums to enhance certain cell functions such as growth. Effects of medium optimization will be measured using cell viability assays, immunofluorescence microscopy and RNA-Seq. The candidate will furthermore design a rulebook for medium optimization and cell culture methods for static, dynamic, 2D and 3D cell culture.
Responsibilities and qualifications
The purpose of the PhD is to gather information about how various cell types use medium components such as amino acids and glucose and to which extent cells are producing metabolites.
These measurements will form the basis for rational design of optimized mediums.
- Cell culture of various cancer and stem cells lines including maturation and differentiation
- NMR analysis of medium usage
- Create an algorithm for medium optimization based on the usage pattern.
- Test mediums in static and dynamic cultures in 2D or 3D
- Analyze cell culture endpoints such as cell growth or differentiation using immunofluorescence and RNA-Seq
- Go to our collaborator at Amsterdam Medical School to culture advanced stem cells culture including the intestine, blood brain barrier and brain organoids.
We are looking for a candidate with a background in biology or bioengineering. Specific qualification are:
- Practical experience with culturing various mammalian cell types for example stem cells and their differentiation as well as primary and cancer cell cultures.
- Knowledge of the mammalian metabolism
- Practical experience with either NMR analysis, immunofluorescence or RNA-Seq
You must have a two-year master's degree (120 ECTS points) or a similar degree with an academic level equivalent to a two-year master's degree.
Approval and Enrolment
The scholarship for the PhD degree is subject to academic approval, and the candidate will be enrolled in one of the general degree programmes at DTU. For information about our enrolment requirements and the general planning of the PhD study programme, please see DTU's rules for the PhD education
.
We offer
DTU is a leading technical university globally recognized for the excellence of its research, education, innovation and scientific advice. We offer a rewarding and challenging job in an international environment. We strive for academic excellence in an environment characterized by collegial respect and academic freedom tempered by responsibility.
Salary and appointment terms
The appointment will be based on the collective agreement with the Danish Confederation of Professional Associations. The allowance will be agreed upon with the relevant union. The period of employment is 3 years. Starting date: not later than 1 January 2024.
You can read more about career paths at DTU here http://www.dtu.dk/english/about/job-and-career/working-at-dtu/career-paths.
Further information
Further information may be obtained from Martin Dufva (dufva@dtu.dk
) (+45 51 33 37 53).
You can read more about DTU Health Tech at www.healthtech.dtu.dk/english.
If you are applying from abroad, you may find useful information on working in Denmark and at DTU at DTU – Moving to Denmark
. Furthermore, you have the option of joining our monthly free seminar “PhD relocation to Denmark and startup “Zoom” seminar
” for all questions regarding the practical matters of moving to Denmark and working as a PhD at DTU.
Application procedure
Your complete online application must be submitted no later than 17 October 2023 (23:59)
. Applications must be submitted as one PDF file
containing all materials to be given consideration. To apply, please open the link "Apply now", fill out the online application form, and attach all your materials in English in one PDF file
. The file must include:
- A letter motivating the application (cover letter)
- Curriculum vitae
- Grade transcripts and BSc/MSc diploma (in English) including official description of grading scale
You may apply prior to obtaining your master's degree but cannot begin before having received it.
Applications received after the deadline will not be considered.
All interested candidates irrespective of age, gender, race, disability, religion or ethnic background are encouraged to apply.
DTU Health Tech engages in research, education, and innovation base on technical and natural science for the healthcare sector. The Healthcare sector is a globally expanding market with demands for the most advanced technological solutions. DTU Health Tech creates the foundation for companies to develop new and innovative services and products which benefit people and create value for society. DTU Health Tech’s expertise spans from imaging and biosensor techniques, across digital health and biological modelling, to biopharma technologies. The department has a scientific staff of about 210 persons, 140 PhD students and a technical/administrative support staff of about 160 persons, of which a large majority contributes to our research infrastructure and related commercial activities.
Technology for people
DTU develops technology for people. With our international elite research and study programmes, we are helping to create a better world and to solve the global challenges formulated in the UN’s 17 Sustainable Development Goals. Hans Christian Ørsted founded DTU in 1829 with a clear mission to develop and create value using science and engineering to benefit society. That mission lives on today. DTU has 13,500 students and 6,000 employees. We work in an international atmosphere and have an inclusive, evolving, and informal working environment. DTU has campuses in all parts of Denmark and in Greenland, and we collaborate with the best universities around the world.
INFORMATIONER OM STILLINGEN:
- Arbejdspladsen ligger i:
Lyngby-Taarbæk Kommune
-Virksomheden tilbyder:
-Arbejdsgiver:
Danmarks Tekniske Universitet, Anker Engelunds Vej, 2800 Kongens Lyngby
-Ansøgning:
Ansøgningsfrist: 17-10-2023; - ansøgningsfristen er overskredet
Se mere her: https://job.jobnet.dk/CV/FindWork/Details/5914976
